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Furthermore, the protein concentration in the supernatant was measured via a BCA protein assay kit (Elabscience, Wuhan, Hubei, China)

Step 2 Membrane Binding and Insertion: Upon binding to membrane-localised HDM-2, PNC-27 inserts into the lipid bilayer via its C-terminal transmembrane-penetrating domain, anchoring the PNC-27HDM-2 complex within the cell membrane